Published: 2018-03-23

Spectrophotometric assessment of effect of aqueous extract of Aloe vera on rabbit erythrocytes in varying concentrations of saline

Vikalp Tiwari, Ashutosh Tiwari, Pooja Solanki Mishra, Vatsala Maheshwari


Background: Hemolytic disorders are one of the prime reasons for frequent blood transfusions which involves lots of costs and sufferings to the patient. This study was undertaken to determine the effect of water soluble extract of Aloe vera on rabbit erythrocytes in varying concentrations of NaCl from 0.9% (isotonic) to 0.15% (hypotonic).

Methods: Aqueous extract of Aloe vera (AVE) 200mg/kg was orally administered to rabbits in the test group while control group was given 1ml of distilled water (DW). Blood was withdrawn from rabbits, centrifuged and suspension in 1ml of normal saline was made. 20 microliter of red blood cells suspension from both control and test groups was added to normal saline of varying concentrations from 0.9% to 0.15% NaCl which were quantitatively analysed for hemolysis by UV spectrophotometer. Data was analysed by unpaired t test and P <0.05 was considered statistically significant.

Results: The difference in percentage of hemolysis in both test and control groups was not statistically significant. Therefore, acute administration of water soluble extract of Aloe vera (200mg/kg) did not have protective effect on rabbit erythrocytes against hypotonic solution of normal saline.

Conclusions: Aloe vera might be useful for the treatment of oxidative stress-related human disorders by virtue of its antioxidant activity and may have a role in prevention of hemolysis which needs to be explored by further studies.


Aloe vera, Antioxidant, Erythrocytes, Hemolysis, Hypotonic saline

Full Text:



Ballabh B, Chaurasia OP. Traditional medicinal plants of cold desert Ladakh-Used in treatment of cold, cough and fever. Journal of Ethnopharmacology. 2007;112(2):341-5.

Rajeswari R, Umadevi M, Rahale CS, Selvavenkadesh S, Kumar KS, Bhowmik D. Aloe vera: the miracle plant its medicinal and traditional uses in India. Journal of Pharmacognosy and Phytochemistry. 2012 Nov 1;1(4).

Eggli U. Illustrated Handbook of Succulent plants: Monocotyledons. Springer; 2001:102-186.

Yates A. Yates Garden Guide. Harper Collins (Australia) Pvt Ltd. 2002:466.

Yamaguchi I, Mega N, Sanada H. Components of the Gel of Aloe vera (L.) Bunn. f. Bioscience, biotechnology, and biochemistry. 1993 Jan 1;57(8):1350-2.

Rishi P, Rampuria A, Tewari R, Koul A. Phytomodulatory potentials of Aloe vera against Salmonella OmpR‐mediated inflammation. Phytotherapy research. 2008 Aug 1;22(8):1075-82.

Tian B, Hua YJ, Ma XQ, Wang GL. Relationship between antibacterial activity of Aloe and its anthraquinone compounds. Zhongguo Zhong Yao Za Zhi. 2003;11:1034-7.

Jasso de Rodriguez D, Hernández-Castillo D, Rodriguez-García R, Angulo-Sánchez JL. Antifungal activity in vitro of Aloe vera pulp and liquid fraction against plant pathogenic fungi. Industrial Crops and Products. 2005;21:81-7.

Hu Y, Xu J, Hu Q. Evaluation of Antioxidant Potential of Aloe vera (Aloe barbadensis Miller) Extracts. Journal of Agricultural and Food Chemistry. 2003;51(26):7788-91.

Pecere T, Gazzola MV, Mucignat C, Parolin C, Vecchia FD, Cavaggioni A, et al. Aloe-emodin is a new type of anticancer agent with selective activity against neuroectodermal tumors. Cancer Research. 2000;60:2800-4.

OECD/OCDE, OECD Guidelines for the testing of chemicals, revised draft guidelines 423: Acute Oral toxicity- Acute toxic class method, revised document, CPCSEA, Ministry of Social Justice and Empowerment, Govt. of India; 2000.

Olaifa AK. Effects of Aloe vera gel application on epidermal wound healing in the domestic rabbit. Int J Res Med Sci. 2017;5:101-5.

Mazzulla S, Sesti S, Schella A, Perrotta I, Anile A, Drogo S. Protective effect of Aloe vera (Aloe barbadensis Miller) on erythrocytes anion transporter and oxidative change. Food and Nutrition Sciences. 2012 Dec 10;3(12):1697.

Kammoun M, Miladi S, Ali YB, Damak M, Gargouri Y, Bezzine S. In vitro study of the PLA2 inhibition and antioxidant activities of Aloe vera leaf skin extracts. Lipids in health and disease. 2011 Dec;10(1):30.

Nwaoguikpe RN, Braide W, Ezejiofor TIN. The effect of aloe vera plant (aloe barbadensis) extracts on sickle cell blood (hbss). African Journal of Food Science and Technology. 2010;1(3):058-63.