Published: 2019-12-24

Evaluation of antidepressant activity of hydro-alcoholic extract of rhizomes of Nardostachys jatamansi DC per se and in combination with fluoxetine in wistar albino rats and swiss albino mice

Radhika Panchal, Nutanbala N. Goswami, Ashish Anovadiya


Background: Depression is a common mental disorder results due to deficiency of neurotransmitter in the brain. Various medicinal properties of jatamansi are mentioned in Ayurveda. This study evaluated effect of hydro-alcoholic extract of rhizomes of Nordostachys jatamansi DC per se and in combination with fluoxetine in wistar albino rats and swiss albino mice.

Methods: Animals of either sex were selected and randomly divided in test group. Jatamansi extract 10:1 and fluoxetine hydrochloride dissolved in distilled water were used. Animals were tested for forced swimming test, tail suspension test and locomotor after given test drug. Results were compared with control and analysed.

Results: Nardostachys jatamansi DC, when given to rats showed dose dependent increase in number of rotation during forced swimming test in rats. During forced swimming test in glass jar statistically significant decrease in immobility was observed. Nardostachys jatamansi DC, when given to mice dose dependent statistically significant decrease in immobility time, swimming time and climbing observed. When given along with combination of fluoxetine it shows statistically significant difference in result, confirmed that it can have synergistic antidepressant activity. When used for locomotor activity in mice none of the test drugs significantly increase or decrease the locomotor activity.

Conclusions: Jatamansi showed antidepressant like property in various tests conducted on rats and mice. It showed statistically significant result with increasing dose and had synergic effect when given along with fluoxetine.


Forced swim test, Tail suspension test, Depression, Jatamansi

Full Text:



WHO. Risks to mental health: An overview of vulnerabilities and risk factors. J Psychiatr Res. 2012: 1-14.

Cheung IWY, Nakayama S, Hsu MNK, Samaranayaka AGP, Li-Chan ECY. Angiotensin -I converting enzyme inhibitory activity of hydrolysates from oat protein by in silino and vitro analysis. J Agric Food chem. 2009;57(19):234-42.

Souery D, Amsterdam J, deMontigny C, Lecrubier Y, Montgomery S, Lipp O, et al. Treatment resistant depression: methodological overview and operational criteria. Eur Neuropsychopharmacol. 1999;9:83-91.

Lee G, Bae H. Therapeutic effects of Phytochemicals and Medicinal Herbs in Depression. Biomed Res Int. 2010;6(3):205-12

Sahu R, Dhongade HJ, Pandey A, Sahu P, Patel D. Medicinal properties of Nardostachys Jatamansi. Oriental J Chem. 2016;32(2):859-66

Rahman H, Muralidharan P. Comparative study of antidepressant activity of methanolic extract of Nardostachys Jatamansi DC Rhizome on normal and sleep deprived mice. Scholars Res Library Der Pharmacia Lettre. 2010;2(5):441-9.

Nomura S, Shimizu J, Kinjo M, Kametani H, Nakazawa T. A new behavioral test for antidepressant drugs. Eur J Pharmacol. 1982;83(3-4):171-5.

Can A, Dao DT, Arad M, Terrillion CE, Piantadosi SC, Gould TD. The Mouse Forced Swim Test. J Vis Exp. 2012;59:3638.

Porsolt RD, Anton G, Blavet N, Jalfre M.Behavioural despair in rats: a new model sensitive to antidepressant treatments. Eur J Pharmacol. 1978;47(4):379-91.

Steru L, Chermat R, Thierry B, Simon P.The tail suspension test: a new method for screening antidepressants in mice. Psychopharmacology (Berl). 1985;85(3):367-70.

Tatem KS, Quinn JL, Phadke A, Yu Q, Gordish-Dressman H, Nagaraju K. Behavioral and Locomotor Measurements Using an Open Field Activity Monitoring System for Skeletal Muscle Diseases. J Vis Exp. 2014;91:51785.

Tripathi KD. Drug used in mental illness. Chapter 33, Essential of Medical Pharmacology, 8th edition. Jaypee Brothers Medical publishers; 2016.

Vogel HG, Vogel WH. Drug discovery and evaluation: Pharmacological assays. Springer Sci Business Media; 2013.

Deepa B, Suchetha K, Rao S. Antidepressant activity of nardostachys jatamansi in electron beam irradiated mice. Int J Res Ayurveda Pharmacy. 2013;4(1):101-3.

Dhingra D, Goyal PK. Inhibition of MAO and GABA: probable mechanisms for antidepressant-like activity of Nardostachys jatamansi DC. in mice. Indian J Exp Biol. 2008;46(4):212-8.